We use a program (KaleidaGraph, Synergy Software ) to perform a non-linear regression on the standard curve data to get the five parameters (A, B, C, D, and E in the formula shown here) .
Description: We use a program (KaleidaGraph, Synergy Software ) to perform a non-linear regression on the standard curve data to get the five parameters (A, B, C, D, and E in the formula shown here) .